type: BadgeClass
id: https://api.badgr.io/public/badges/lnAeMeWyTsK0MoTa_Ta7-A
name: Constructing Fluorescent Plasmids: Participant
image:
  id: https://badges.parchment.com/public/badges/lnAeMeWyTsK0MoTa_Ta7-A/image
image alt text: Data & AI Literacy in Action badge image
description: Earners of this badge participated in the Wet Lab Makerspace’s Spring 2026 Community Project: Constructing Fluorescent Plasmids. This was a non-credit course in which students of any disciplinary background could enroll to learn foundational laboratory skills. They were introduced to a workflow for Gibson assembly of fluorescent plasmids and attempted to cultivate bacteria and perform DNA extraction, PCR, gel electrophoresis, Gibson assembly, and colony PCR.
issuer: https://api.badgr.io/public/issuers/WOyxSKfwRSO0vy8BQ_i7Ig
tags: #northeasternuniversity
tags: wet lab makerspace
tags: wet lab makerspace community project
tags: level 1
tags: spring 2026
alignment:
  target name: laboratory safety
  target url: https://lightcast.io/open-skills/skills/ES1D5B4B968B9AA7BF21/laboratory-safety
alignment:
  target name: bacterial cultures
  target url: https://lightcast.io/open-skills/skills/KS120W16P3YDQCK67JGD/bacterial-cultures
alignment:
  target name: molecular cloning
  target url: https://lightcast.io/open-skills/skills/KS120W26TFXN7GRHV8LJ/molecular-cloning
alignment:
  target name: polymerase chain reaction
  target url: https://lightcast.io/open-skills/skills/KS127MQ6GC1CMJ5B0737/polymerase-chain-reaction
alignment:
  target name: DNA extraction
  target url: https://lightcast.io/open-skills/skills/KS1232Y77JCZ23Q3BTL5/dna-extraction
criteria:
  narrative: - Complete in-person laboratory safety training. - Read provided background information and protocols for plasmid assembly and transformation Basic Workflow. - Pass all quizzes on background information with scores of 80% or higher.
extra description:
  type: Extension
  type: extensions:ExtraDescriptionExtension
  name: Learn More
  narrative: This badge may be earned by NU undergraduate and graduate students who participate in the Wet Lab Makerspace’s Spring 2026 Community Project. Visit the Wet Lab Makerspace [website]( https://makerspaces.northeastern.edu/spaces/wet-lab-makerspace/) to learn more.
human code: INPR0400