Constructing Fluorescent Plasmids: Advanced Workflow
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Earners of this badge successfully completed both the Basic and Advanced Workflows of the Wet Lab Makerspace’s Spring 2026 Community Project: Constructing Fluorescent Plasmids. This was a non-credit course in which students of any disciplinary background could enroll to learn foundational laboratory skills. They used Gibson assembly to clone a gene encoding a fluorescent protein into a plasmid backbone and transformed that plasmid into E. coli. They then used the skills they learned to clone a chromogenic chromosomal gene from another strain into the same plasmid backbone and transform it into E. coli.
- Complete in-person laboratory safety training. - Read provided background information and protocols for plasmid assembly and transformation Basic Workflow. - Pass all quizzes on background information with scores of 80% or higher. - Create a fluorescent plasmid and transform it into _E. coli_. - Document the completion of all steps in the plasmid assembly and transformation Basic Workflow. - Design primers and clone a chromogenic gene from another strain into _E. coli_. - Document completion of all steps in the plasmid assembly and transformation Advanced Workflow.
This badge may be earned by NU undergraduate and graduate students who participate in the Wet Lab Makerspace’s Spring 2026 Community Project. Visit the Wet Lab Makerspace [website]( https://makerspaces.northeastern.edu/spaces/wet-lab-makerspace/) to learn more.